Medicinal plants are very rich in valuable metabolites. These metabolites will act as drug. Tulasi ssbelongs to one of the medicinal plant. It has both primary and secondary metabolites. A long back they are using for different purposes. It will act as anti microbial, antifungal and anti viral agents for many diseases. The most important thing is it has eugenol which will act as pain killer. In our present study we used main three parts of tulasi based to identify the different metabolites.