The possibility of using autogamous forms of alfalfa in the breeding of varieties with stable seed productivity in the conditions of insufficient number of insect-pollinators has been proved. Aim of present study was to evaluate the breeding value of alfalfa inbred lines with different degree of autogamy for creation of synthetic varieties. Inbred lines of the seventh- tenth generations were obtained with a high level of autogamy (41-76%) and seed yield at the level of the variety – standard. The splitting on the trait takes place among the descendants of the inbred generations on the both of the autogamous and self-incompatible plants. The invariable high-autogamous lines have been not obtained for 34 years of research. The autogamy levels above 40.1%, had 55% of lines in the tenth generation. Inbred lines have been created with forage yields at the standard-grade level and high seed yields. Synthetic varieties with seed yields of 0.300- 0.321 t/ha and different number of components (5-7) were formed on the basis of lines with high combining ability effects. Autogamous forms which were created do not lose the ability to cross pollination.